The Resolute® BioSC System can be a highly modular multi-step chromatography system that will continuously work three chromatography separations (in batch or multi-column method), including viral inactivation As well as in-line buffer preparing. The chaining of numerous device functions with each other ends in a compact and intensified procedure.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
物質の濃度により光の通過する角度が変わることを利用した検出器。原理上グラジェント分析はできない（グラジェントによって移動相自体の屈折率が変化するため）。また、感度が低いのが欠点だが、大部分の物質に対して使用できる。
- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
Samples in liquid kind are injected in to the HPLC after an appropriate clean-up to get rid of any particulate products, or after an acceptable extraction to eliminate matrix interferents. In determining polyaromatic hydrocarbons (PAH) in wastewater, by way of example, an extraction with CH2Cl2 serves the dual intent of concentrating the analytes and isolating them from matrix interferents. Solid samples are very first dissolved in an appropriate solvent or even the analytes of desire introduced into Answer by extraction. One example is, an HPLC Investigation for your Lively elements as well as degradation merchandise in the pharmaceutical tablet often commences by extracting the powdered tablet which has a portion of mobile section.
An interior normal is important when working with HPLC–MS as the interface between the HPLC as well as mass website spectrometer does not make it possible for to get a reproducible transfer on the column’s eluent in the MS’s ionization chamber.
混合物で構成される試料を分離する。一般にステンレス製の筒の中に、微細な真球状の多孔質シリカゲルをアルキル基等で修飾した物を充填して用いる。分取目的であれば、粉砕シリカゲルも用いられる。
The running pressure inside of an HPLC is sufficiently high that we can't inject the sample in to the cell stage by inserting a syringe via a septum, as is feasible in fuel chromatography. As an alternative, we inject the sample employing a loop injector
The data acquisition system information and processes the indicators within the detector, permitting to the generation of chromatograms and the quantification of compounds.
System contamination: Dirty HPLC lines, injectors, or here detectors can introduce contaminants that present up as ghost peaks. Flush the system with appropriate solvents to remove any accumulated contaminants.
, which can be the more popular sort of HPLC, the stationary phase is nonpolar plus the mobile stage is polar. The commonest nonpolar stationary phases use an organochlorosilane the place the R group is undoubtedly an n
There are lots of selections for checking the chromatogram when utilizing a mass spectrometer as the detector. The commonest technique will be to consistently scan your complete mass spectrum and report the total sign for all ions achieving the detector all through Just about every scan. This full ion scan offers common detection for all analytes. As seen in Figure twelve.five.14
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, that's the greater prevalent method of HPLC, the stationary section is nonpolar and also the mobile phase is polar. The most common nonpolar stationary phases use an organochlorosilane wherever the R team is undoubtedly an n